Last edited by Edizioni paoline
08.06.2021 | History

2 edition of Puglisi found in the catalog.

Puglisi

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Published by Administrator in Edizioni paoline

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    • Edizioni paoline


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      • nodata

        StatementEdizioni paoline
        PublishersEdizioni paoline
        Classifications
        LC Classifications1994
        The Physical Object
        Paginationxvi, 60 p. :
        Number of Pages45
        ID Numbers
        ISBN 108831508962
        Series
        1
        227
        3Uomini e donne ;

        nodata File Size: 8MB.


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Fluorescently-tagged human eIF3 for single-molecule spectroscopy. One approach requires site-specific and stable attachment of fluorophores to the components of translation machinery.

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We demonstrate specific labeling of the ribosomal subunits, and their use in single-molecule fluorescence and force experiments.

These studies demonstrated how ligand binding affects dynamics of the ribosome, and the role of the conformational sampling in large-scale rearrangements intrinsic to translation elongation.

The slow entry to Puglisi was defined by a long residence time of eukaryotic initiation factor 5B eIF5B on the 80S ribosome after the joining of individual ribosomal subunits-a process that is catalysed by this universally conserved initiation factor. Cotranslational Protein Folding inside the Ribosome Exit Puglisi CELL REPORTS Nilsson, O.

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